Therapeutic index estimator for frog-derived helical antimicrobial peptides

Please type or paste your single-letter amino acid code for the peptide sequence

Note a): The therapeutic index measured in experiments, which is relevant for comparison to predicted therapeutic index, is defined as TI = HC50/MIC, where HC50 value is the mean concentration of peptide producing 50% hemolysis of freshly isolated human erythrocytes from healthy donors, while MIC is defined as the minimum inhibitory concentration using the microdilution susceptibility test against Escherichia coli.

Note b): Predicted TI values are only estimates and should not be used outside above mentioned range of frog-derived helical peptides. Within that range calculated TI values by the deserv1 can not be used to distinguish peptide hemolytics (toxins) from mediocre peptide antibiotics with low selectivity. The best usage of calculated TI values is for frog-derived peptide antibiotics, expected to have high selectivity, as fast and easy confirmation that such expectations are well enough grounded to justify synthesis and testing of modified peptides.

Note c) When you use or mention this server in a publication please cite the reference:
Davor Juretić, Damir Vukičević, Nada Ilić, Nikolinka Antcheva and Alessandro Tossi: “ Computational Design of Highly Selective Antimicrobial Peptides” J. Chem. Inf. Model. 49, 2873-2882 (2009).

This web service is written by Viktor Bojovic (